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DNA aptamers recognizing the histidine tag and their use

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Prokaryotic and eukaryotic expression systems for the production of recombinant proteins are used on a large scale in scientific research. The genetically engineered fusion tags, e.g. histidine tag (His-Tag) are used for fast and efficient purification
of the desired recombinant proteins. The high affinity of His-Tag to the certain metal ions (cobalt, copper, nickel or zinc) enables the application
of chromatographic media with the above mentioned ions for the purification
or immobilization of His-Tagged proteins.

His-Tag recombinant protein can be detected in western blotting using anti-His mono- and polyclonal antibodies. Short His-Tag can be used to investigate protein-protein interactions in techniques such as pull down assays, immunoprecpitation, co-immunoprecipitation or Far-Western blot analysis.

DNA aptamers are single-stranded DNA oligonucleotides of approximately 4-100 nucleotides that exhibit a high affinity and specificity to their corresponding ligand. An optimal binding between target molecule and selected aptamer is possible due to the rich secondary and tertiary structures of the latter one.

The subject of the offer are unique sequences of single-stranded DNA aptamers that recognize His-Tag and their application in biotechnology in analysis of His-Tagged molecules (peptides, proteins or DNA derivatives).

The offered DNA aptamers can be used for analysis of molecules containing a His-Tag, and in particular to:
• application as a agonist for anti-histidine antibodies;
• purification and detection of the target molecules containing a His-Tag.

In addition to the above applications, DNA aptamers can be used as molecules
with attached tags, especially fluorescent, and in particular to label the molecular targets containing His-Tag. This type of molecules can be successfully used e.g.
to study the interaction between proteins using spectroscopic techniques.

Financial information

The offered Anti-Histidine Tag DNA aptamers for biotechnological applications
is patent pending. Further research and development of the invention
are continued at the Faculty of Biochemistry, Biophysics and Biotechnology
of the Jagiellonian University. Currently the Centre for Innovation, Technology Transfer and University Development (CITTRU) is looking for entities interested
in commercial application of the invention.

Patent publications:

PL 403939

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klodette (Poland)

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